Protein Method Blocking Buffers
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (10X, with 1% Tween-20, pH 7.4) - 1L
10X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml) - MB-070-010
Western Blot Blocking Buffer is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. This buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. See www.rockland-inc.com for specific protocols. This product is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Biogenex Laboratories PROTEIN BLOCK GOAT SERUM 50ML
Protein block/normal goat serum (for use with mouse and rabbit systems) 50mL
IBI SCIENTIFIC 3P 10X PBST (PBS W/0.5% TWEEN 20) 6/PACK
Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.5% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 10X PBST (10X PBS) with TWEEN 20 (0.5% TWEEN 20) is a concentrated sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 10X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI.
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (10X, with 0.5% Tween-20, pH 7.6) - 1L
10X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Biogenex Laboratories Power Block
Power Block ™ reagent is a highly effective blocking reagent capable of reducing non-specific background staining associated with immunodetection techniques such as immunocytochemistry, ELISA, and Western blotting. Antibodies tend to bind non-specifically to membranes and tissues. To minimize this nonspecific binding, a blocking buffer is used to enhance signal-to noise ratio. Power Block™ reagent shows no cross-reactivity and it blocks more effectively than serum albumins. The blocking potency of Power Block™ reagent is superior to most other conventional formulations in producing a high signal-to-noise ratio. Power Block™ reagent may also be used as effective diluents for antibodies.""""
SCANTIBODIES LABORATORY INC NON-SPECIFIC ANTIBODY BLOCKING TUBE (31X761) 50PK
The 31X761 NABT (Non-Specific Antibody Blocking Tube) from Scantibodies Laboratory, Inc. contains immunoglobulins which the non-specific antibodies in the serum or plasma samples bind to, thus causing them to be blocked from interfering in antibody detection immunoassays. The NABT also allows for the rapid and simple elimination of false positive non-specific antibody interference in plasma or serum for detection assays (i.e., anti-HCV, HIV, Toxoplasmosis, Rubella, CMV, Herpes, Tg, Thyroglobulin, TPO Thrombopoietin, etc.). The reagent is in the form of a lyophilized pellet at the bottom of the tube and each tube contains enough reagents to inactivate the non-specific antibodies in 500 μl of sample material.
LI COR BIOTECH LLC TBS Blocking Buffer and PVDF Kits (single buffer kit)
The non-mammalian Intercept Blocker in these kits is in TBS (Tris-buffered saline) containing 0.06% Kathon II. The Immobilon-FL PVDF membrane included in these kits has been tested to ensure a low background for infrared Western blot success. This kit contains Intercept (TBS) Blocking Buffer (500 mL) and Millipore Immobilon-FL PVDF Membrane (1 roll; 0.45µm, 26.5 cm x 3.75 m).
Abcam ELISA Coating Buffer 1X, 120ML
ELISA Coating Buffer 1X (ab210899) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack
Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack
Santa Cruz Biotechnology UltraCruz Blocking Reagent, 100 mL
Plant protein derived universal antibody diluent and blocking reagent recommended for use in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and for other research applications. Animal-free alternative to sera, BSA or non-fat dry milk. Designed to stabilize diluted antibody solutions and minimize background due to non-specific binding in different antibody-based research applications. The UltraCruz Blocking Reagent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.