Protein Method Blocking Buffers
Biolegend Tris-Buffered Saline 20X (1.0 M) w/ Tween 20
Tris-Buffered Saline 20X (1.0 M) w/ Tween 20 Apps: IHC; Size: 500 mL
Biotium Permeabilization and Blocking Buffer (5X)
Concentrated buffer stock solution (5X) for one step permeabilization and blocking for immunofluorescence staining. Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies. We recommend Permeabilization Buffer for use with anti-goat and anti-sheep secondary antibodies.
Biogenex Laboratories PROTEIN BLOCK GOAT SERUM 50ML
Protein block/normal goat serum (for use with mouse and rabbit systems) 50mL
Biogenex Laboratories Power Block
Power Block ™ reagent is a highly effective blocking reagent capable of reducing non-specific background staining associated with immunodetection techniques such as immunocytochemistry, ELISA, and Western blotting. Antibodies tend to bind non-specifically to membranes and tissues. To minimize this nonspecific binding, a blocking buffer is used to enhance signal-to noise ratio. Power Block™ reagent shows no cross-reactivity and it blocks more effectively than serum albumins. The blocking potency of Power Block™ reagent is superior to most other conventional formulations in producing a high signal-to-noise ratio. Power Block™ reagent may also be used as effective diluents for antibodies.""""
Teknova 20X PBS with 1.0% Tween-20. 20L, Sterile
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
IBI SCIENTIFIC 3P 10X PBST (PBS W/0.5% TWEEN 20) 4/PACK
"Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.5% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 10X PBST (10X PBS) with TWEEN 20 (0.5% TWEEN 20) is a concentrated sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 10X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI."
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (10X, with 0.5% Tween-20, pH 7.4) - 4x1L
10X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Carbosynth LLC. HAMA blocking reagent, 1 gram
HAMA blocking reagent for use in assays specific for clinical false positive samples
Seracare Life Sciences Inc 10% BSA DILUENT/BLOCKING SOL
Convenient antibody diluent/blocking solution concentrate containing 10% bovine serum albumin in phosphate buffer. Compatible with most assay formats.
Bioworld Casein-Blocking Buffer in PBS, 500 mL
Product DescriptionCasein-blocking buffer in PBS is a purified casein protein solution used for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods. Phosphate-buffered saline formulations are used for blocking excess binding sites on membranes and microplates in antibody-based detection methods.This blocking buffer was purified from milk and contains 1% (w/v) casein that corresponds to the optimal concentration for most applications.
Bioworld Blotto/Tween in PBS, 250 mL
Blotto/Antifoam in PBS Blot blocking buffer used to block non-specific binding site for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.05% Tween, 0.02% Sodium azide in PBS
Cell Signaling Technology Phosphate Buffered Saline with Tween 20, 20X Concentration, 1000 mL
Phosphate Buffered Saline with Tween™ 20 (PBST-20X) is a 20X concentrated buffer solution formulated for use as a wash buffer and diluent for ELISA applications. The 1X working solution contains 3.2 mM Na2HPO4, 0.5 mM KH2PO4, 1.3 mM KCl, 135 mM NaCl, and 0.05% Tween™ 20 at pH 7.4.
- Supplied as a 20X concentrated solution
- Yields 20 L of 1X buffer when diluted with ddH2O
- Formulated for use in ELISA
- Stable for 24 months when stored at room temperature
Nacalai USA Inc NACALAI USA INC HPLC Column, 4.6 mm I.D., 150 mm, 3 μm Particle Size
- Reversed-phase HPLC column with naphtylethyl group bonded silica
- Designed for structural isomers and unsaturated compounds
- Strong π-π interactions, superior to phenyl columns
- Separates tolunitrile isomers, steroids, tocopherols, cisplatin variants
- High purity porous spherical silica gel
- 4.6 mm I.D. x 150 mm, 3 μm particle size, Waters type end-fitting
- Average pore size: 120 Å
- Specific surface area: 300 m²/g
- Carbon content: 11%
- Usable pH range: 2 to 7.5
- Maximum pressure: 15MPa
- Maximum usable temperature: 60°C
LICORbio Intercept (PBS) Protein-Free Blocking Buffer (500 mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.